云南滇黄精药渣中多糖含量的测定#br#

doi:10.3969/j.issn.1009-0479.2022.01.018

昆明冶金职业大学学报 ›› 2022, Vol. 38 ›› Issue (1): 101-.DOI: 10.3969/j.issn.1009-0479.2022.01.018

云南滇黄精药渣中多糖含量的测定#br#

姚波，黄力，李冬丽

(昆明冶金高等专科学校环境与化工学院，云南昆明650033)

收稿日期:2021-10-15 出版日期:2022-02-14 发布日期:2023-12-04
作者简介:姚波（１９８３－），女，云南石屏人，讲师，理学硕士，主要从事化工生物技术专业教学与研究。
基金资助:
昆明冶金高等专科学校科研基金项目：利用云南滇黄精药渣制备膳食纤维的研究（２０２０ＸＪＺＫ０２）。

Determination of Polysaccharide Content in the Residue of Polygonatum kingianum in Yunnan Province

YAO Bo，HUANG Li，LI Dongli

(Faculty of Environmental and Chemical Engineering, Kunming Metallurgy College , Kunming 650033, China)

Received:2021-10-15 Online:2022-02-14 Published:2023-12-04

摘要/Abstract

摘要： 以测定云南滇黄精药渣中多糖的含量为目的，采用热回流法提取总多糖，以葡萄糖为对照品制作标准曲线，采用苯酚-硫酸法和可见分光光度法测定多糖含量。研究结果表明该方法的线性关系良好，线性相关系数20.999，精密度良好，相对标准偏差≤3%，准确性良好，平均回收率为99.40%，测得6 批滇黄精药渣中多糖的平均含量为 4.47% ，实验结果可为滇黄精的综合利用提供一定的参考。

关键词: 滇黄精, 药渣, 多糖, 含量测定

Abstract:

In order to determine the content of polysaccharide in the residue of polygonatum kingianumfrom Yunnan Province, the total polysaccharides was extracted by thermal reflux method, and the stand-ard curve was obtained with glucose as control. The polysaccharide content was determined by sulfuricacid-phenol and ultraviolet spectrophotometer. The results show that the linear correlation coefficient wasnot less than 0. 999% , the relative standard deviation was not more than 3% , and the accuracy wasgood. The average recovery rate was 99. 40% . The average content of polysaccharide in the six batches otsamples was 4.47% and the experimental results can provide some reference for comprehensive utilization of polygonatum kingianum.

Key words: polygonatum kingianum, residue, polysaccharide, determination

中图分类号:

TQ460.7⁺2

姚波, 黄力, 李冬丽. 云南滇黄精药渣中多糖含量的测定#br#[J]. 昆明冶金职业大学学报, 2022, 38(1): 101-.

YAO Bo, HUANG Li, LI Dongli. Determination of Polysaccharide Content in the Residue of Polygonatum kingianum in Yunnan Province[J]. HPMug2oMmNrOfxWQHLiEksa6s0hFu9Ox348d7QefarYlaFR5ArkhOwm3Da1pmxmxCtenj1+6luWD#r#n+EPn9L6Ce+9onqnMlT+i, 2022, 38(1): 101-.

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云南滇黄精药渣中多糖含量的测定#br#

Determination of Polysaccharide Content in the Residue of Polygonatum kingianum in Yunnan Province

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