关键词: 羊耳菊, 产地, 总酚, 含量测定

Abstract:

A method for determination of the total phenolic content of Inula cappa was established.andthe amount of total phenol in nula cappa from different production areas in Yunnan was compared. Theextraction technology was optimized through single factor test and orthogonal testand the content of totalphenolin Inula cappa was determined by visible spectrophotometry with thymol as control substance. Theresults showed that the maximum absorbance of thymol solution was obtained at the wavelength of278 nm , and there was a good linear relationship between the concentration and absorbance in the rangeof 9. 8 ~ 68. 6 mg/L (r' =0. 999 2). The precision RSD of the method was 1. 45% , and the average re-covery rate was 98. 62% . The analvsis method was stable and reliable. The content of total phenol in 9batches from different producing areas was different, ranging from 7. 61 ~2. 89 mg/g. The experimentalresults can provide some reference for improving the quality standard and further developing and utilizingthe products of Inula cappa.

Key words: Inula cappa, origin, total phenol, content determination